Journal: Cancers

Article Title: Regulation of KIF2A by Antitumor miR-451a Inhibits Cancer Cell Aggressiveness Features in Lung Squamous Cell Carcinoma

doi: 10.3390/cancers11020258

Figure Lengend Snippet: Cell migration and invasion assays following ectopic expression of miR-451a in LUSQ cells. ( A ) Cell migration was measured by wound healing assays (* p < 0.001). ( B ) Cell invasion was determined by Matrigel invasion assays (* p < 0.001). Phase-contrast micrographs of LUSQ cells in migration and micrographs of LUSQ cells in invasion assays are shown.

Article Snippet: Cell invasion ability was determined with Corning Matrigel Invasion Chambers (Discovery Labware, Inc., Bedford, MA, USA).

Techniques: Migration, Expressing

Journal: Cancers

Article Title: Regulation of KIF2A by Antitumor miR-451a Inhibits Cancer Cell Aggressiveness Features in Lung Squamous Cell Carcinoma

doi: 10.3390/cancers11020258

Figure Lengend Snippet: Effects of KIF2A silencing on cell migration and invasive abilities in LUSQ cells. ( A ) Cell migration was measured by wound healing assays (* p < 0.001). ( B ) Cell invasion was determined by Matrigel invasion assays (* p < 0.001).

Article Snippet: Cell invasion ability was determined with Corning Matrigel Invasion Chambers (Discovery Labware, Inc., Bedford, MA, USA).

Techniques: Migration

Journal: Pharmaceutics

Article Title: A Novel 2-Methoxyestradiol Derivative: Disrupting Mitosis Inhibiting Cell Motility and Inducing Apoptosis in HeLa Cells In Vitro

doi: 10.3390/pharmaceutics16050622

Figure Lengend Snippet: Compound 4a markedly diminished the invasiveness of cervical cancer cells (HeLa). The anti-invasive potential of the test compound is illustrated by representative images ( B ), and is quantified by the percentage of invading cells in the Boyden chamber containing different concentrations of 4a , using an EMEM medium supplemented with 10% FBS as a chemoattractant ( A ). Results are presented as mean values ± SEM of the data from three separate measurements with duplicates. ** and *** indicate p < 0.01 and p < 0.001, respectively, compared to untreated control samples.

Article Snippet: The polyethylene terephthalate (PET) membrane (8 μm pore size) and the thin layer of the matrigel basement matrix in special Boyden chamber inserts (BioCoatTM Matrigel ® Invasion Chambers, Corning Inc., Corning, NY, USA) were prehydrated (2 h, serum-free EMEM) and placed onto a 24-well plate.

Techniques: Control

Journal: BMC Cancer

Article Title: Distinct roles for the RNA-binding protein Staufen1 in prostate cancer

doi: 10.1186/s12885-021-07844-2

Figure Lengend Snippet: Staufen1 knockdown inhibits Prostate Cancer cell invasion and motility. Motility and Invasion assays were performed following 48 h of Control (CTL) or Staufen1-shRNA (shStau1) expression. a Western blot analysis of Staufen1 expression showing a representative blot of Staufen1 with β-actin as a loading control in PC3 and DU145 cells. Each representative blot is cropped to show an n = 1 for each cell line from their respective full-length blot. Note that the DU145 cells used for Fig. 4 were also used for Fig. experiments and therefore a different representative image from the same blot was selected for each figure. Quantification of n = 4 is represented normalized to CTL. Data are Mean ± SD, One-Sample T-Test, **P < 0.01, ***P < 0.001. b Cells were seeded into transwell chambers containing membranes coated with or without Matrigel and incubated for 72 h and 24 h for PC3 and DU145 cells, respectively. Representative images of cells that passed through the transwell chamber at 40X magnification, scale bar = 20 μm, are displayed. Average number of cells/field of view is quantified for cell motility (no matrigel) and invasion (matrigel) as a percentage relative to CTL. Quantifications are for all data are n = 4. Data are Mean ± SD, Students T-Test, **P < 0.01, ***P < 0.001. Full length blots are presented in Supplemental Fig.

Article Snippet: Infected cells (2.5 × 10 4 ) were seeded in serum free medium in Corning ® BioCoatTM Control Inserts (#354578) or Corning ® GFR Matrigel ® Basement Membrane Matrix Invasion Chambers (#354480) (VWR International, Ontario, Canada) containing growth medium in the bottom chamber.

Techniques: Knockdown, Control, shRNA, Expressing, Western Blot, Incubation

Journal: Pharmaceutics

Article Title: Effects of Lactate Transport Inhibition by AZD3965 in Muscle-Invasive Urothelial Bladder Cancer

doi: 10.3390/pharmaceutics15122688

Figure Lengend Snippet: Effect of AZD3965 on the migration ( A ) and invasion ( B ) abilities of HT1376R and 253J urothelial bladder carcinoma cell lines, detected by the wound-healing assay at 24 and 48 h post-treatment ( A ), and by matrigel invasion chambers at 72 h post-treatment ( B ). Representative pictures of the assays for each condition are shown. * p < 0.05, ** p < 0.01 and *** p < 0.005 for AZD3965 treatment versus control condition.

Article Snippet: Invasion capacity of HT1376R and 253J cells was evaluated using a 12-well Transwell invasion assay in BioCoat™ Matrigel ® Invasion Chambers (Corning ® , New York, NY, USA).

Techniques: Migration, Wound Healing Assay